Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

4RJF

Crystal structure of the human sliding clamp at 2.0 angstrom resolution

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2014-07-22
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.5418
Spacegroup nameP 3
Unit cell lengths142.970, 142.970, 41.410
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution39.272 - 2.007
R-factor0.1546
Rwork0.148
R-free0.18870
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1axc
RMSD bond length0.002
RMSD bond angle0.631
Data reduction softwareMOSFLM
Data scaling softwareAimless (0.1.27)
Phasing softwarePHASER (2.5.1)
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]41.41041.4102.060
High resolution limit [Å]2.0079.1902.007
Rmerge0.0550.0240.449
Total number of observations487928350
Number of reflections63388
<I/σ(I)>2583.83.8
Completeness [%]100.098.999.9
Redundancy7.17.46.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72890.08M strontium chloride hexahydrate, 0.02M magnesium chloride hexahydrate, 0.04M sodium cacodylate trihydrate (pH7.0), 20% v/v (+/-)-2-methyl-2,4-pentanediol and 0.012M spermine tetrahydrochloride, final [PCNA] = 11.6mg/mL = 0.40mM, VAPOR DIFFUSION, SITTING DROP, temperature 289K

217705

PDB entries from 2024-03-27

PDB statisticsPDBj update infoContact PDBjnumon