4RJF

Crystal structure of the human sliding clamp at 2.0 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-07-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 3
Unit cell lengths	142.970, 142.970, 41.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.272 - 2.007
R-factor	0.1546
Rwork	0.148
R-free	0.18870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1axc
RMSD bond length	0.002
RMSD bond angle	0.631
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.410	41.410	2.060
High resolution limit [Å]	2.007	9.190	2.007
Rmerge	0.055	0.024	0.449
Total number of observations		4879	28350
Number of reflections	63388
<I/σ(I)>	25	83.8	3.8
Completeness [%]	100.0	98.9	99.9
Redundancy	7.1	7.4	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.08M strontium chloride hexahydrate, 0.02M magnesium chloride hexahydrate, 0.04M sodium cacodylate trihydrate (pH7.0), 20% v/v (+/-)-2-methyl-2,4-pentanediol and 0.012M spermine tetrahydrochloride, final [PCNA] = 11.6mg/mL = 0.40mM, VAPOR DIFFUSION, SITTING DROP, temperature 289K