4RFE
Crystal structure of ADCC-potent ANTI-HIV-1 Rhesus macaque antibody JR4 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-28 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0452 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.418, 79.585, 82.042 |
| Unit cell angles | 78.79, 82.89, 65.17 |
Refinement procedure
| Resolution | 40.200 - 1.910 |
| R-factor | 0.19442 |
| Rwork | 0.192 |
| R-free | 0.24635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tnn |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.774 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.125 | 0.601 |
| Number of reflections | 129457 | |
| <I/σ(I)> | 7.35 | 1.5 |
| Completeness [%] | 93.6 | 95.5 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% PEG 5000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
