4RDQ
Calcium-activated chloride channel bestrophin-1, from chicken, in complex with Fab antibody fragments, chloride and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.540, 242.904, 172.757 |
| Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
| Resolution | 39.891 - 2.850 |
| R-factor | 0.2176 |
| Rwork | 0.217 |
| R-free | 0.23410 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 188162 | |
| <I/σ(I)> | 14.2 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 5% PEG 4000, 75 mM KCl, 75 mM NaCl, 50 mM sodium acetate pH 4.0, 20% glycerol, 0.5 mM Cymal-6-NG (detergent), 10 mM Tris-HCl pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
