4RDN

Structure of YTH-YTHDF2 in complex with m6A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	80.730, 80.730, 113.480
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.030 - 2.100
R-factor	0.2196
Rwork	0.217
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.121
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.914	38.031	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge		0.044	0.535
Total number of observations		6748	29987
Number of reflections	24524
<I/σ(I)>	12	11.5	1.4
Completeness [%]	100.0	99.6	100
Redundancy	8.2	8.4	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	283	0.2 M Potassium tartrate, 0.1 M Sodium citrate tribasic dehydrate, PH 5.6, 2M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 283K