4RDN
Structure of YTH-YTHDF2 in complex with m6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 65 |
Unit cell lengths | 80.730, 80.730, 113.480 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.030 - 2.100 |
R-factor | 0.2196 |
Rwork | 0.217 |
R-free | 0.26990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.121 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.21) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 69.914 | 38.031 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.044 | 0.535 | |
Total number of observations | 6748 | 29987 | |
Number of reflections | 24524 | ||
<I/σ(I)> | 12 | 11.5 | 1.4 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 8.2 | 8.4 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 283 | 0.2 M Potassium tartrate, 0.1 M Sodium citrate tribasic dehydrate, PH 5.6, 2M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 283K |