4RBO

Crystal structure of a Nanog homeobox (NANOG) from Homo sapiens at 3.30 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-22
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 65 2 2
Unit cell lengths	215.603, 215.603, 42.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.899 - 3.300
R-factor	0.2365
Rwork	0.235
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.778
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX ((phenix.refine:)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.899	29.899	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge		0.020	0.552
Total number of observations		301	1939
Number of reflections	8834
<I/σ(I)>	9.4
Completeness [%]	97.0	82.2	94.3
Redundancy	3.4	2.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.66	293	0.200M calcium acetate, 12.00% 2-propanol, 0.1M sodium cacodylate pH 5.66, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K