4R2C
Egr1/Zif268 zinc fingers in complex with hydroxymethylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.977, 56.076, 129.605 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.038 - 1.890 |
| R-factor | 0.2013 |
| Rwork | 0.199 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2a |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.040 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.072 | 0.463 |
| Number of reflections | 13248 | |
| <I/σ(I)> | 29.9 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 35% (v/v) pentaerythritol propoxylate (5/4 PO/OH), 0.2 M KCl, 50 mM Hepes-NaOH, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
