4R0L
Anti-canine CD28 antibody, 1C6, bound canine CD28
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.27 |
Spacegroup name | P 31 |
Unit cell lengths | 86.783, 86.783, 205.804 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.460 - 3.300 |
R-factor | 0.25792 |
Rwork | 0.256 |
R-free | 0.29283 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.391 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.065 | 0.484 |
Number of reflections | 25360 | |
<I/σ(I)> | 15.1 | 2.3 |
Completeness [%] | 96.9 | 97.5 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.9M AmSO4, 0.1M Cesium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |