4R0E
Crystal Structure of the Poliovirus RNA-Dependent RNA Polymerase Low-Fidelity Mutant 3Dpol H273R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-26 |
| Detector | RIGAKU |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 65 |
| Unit cell lengths | 126.440, 126.440, 113.246 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.870 - 3.000 |
| R-factor | 0.18763 |
| Rwork | 0.186 |
| R-free | 0.21767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.467 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.067 | 0.313 |
| Number of reflections | 19450 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | Mixed equal volumes of protein 10mg/ml in buffer (5mM Tris pH 7.5, 200mM NaCl, 0.1mM EDTA, 2mM DTT) and reservoir solution (2M Na Acetate, 0.1 M Na Cacodylate pH 6.8) prior to vapor diffusion, VAPOR DIFFUSION, temperature 293K |
