4R0E

Crystal Structure of the Poliovirus RNA-Dependent RNA Polymerase Low-Fidelity Mutant 3Dpol H273R

Summary for 4R0E

• Entry DOI: 10.2210/pdb4r0e/pdb
• Related: 2IJF
• Descriptor: RNA-directed RNA polymerase (2 entities in total)
• Functional Keywords: rna-dependent rna polymerase, fidelity, low-fidelity, rna polymerase, poliovirus, transferase
• Biological source: Human poliovirus 1
• Cellular location: Capsid protein VP0: Virion. Capsid protein VP4: Virion. Capsid protein VP2: Virion. Capsid protein VP3: Virion. Capsid protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 2C: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3A: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Protein 3AB: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Viral protein genome-linked: Virion. Protease 3C: Host cytoplasm. Protein 3CD: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . RNA-directed RNA polymerase: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : P03300
• Total number of polymer chains: 1
• Total formula weight: 52590.98

Authors

Marcotte, L.L.,Gohara, D.W.,Filman, D.J.,Hogle, J.M. (deposition date: 2014-07-30, release date: 2014-11-12, Last modification date: 2023-09-20)

• Primary citation: Moustafa, I.M.,Korboukh, V.K.,Arnold, J.J.,Smidansky, E.D.,Marcotte, L.L.,Gohara, D.W.,Yang, X.,Sanchez-Farran, M.A.,Filman, D.,Maranas, J.K.,Boehr, D.D.,Hogle, J.M.,Colina, C.M.,Cameron, C.E.; Structural Dynamics as a Contributor to Error-prone Replication by an RNA-dependent RNA Polymerase.; J.Biol.Chem., 289:36229-36248, 2014; PubMed: 25378410; DOI: 10.1074/jbc.M114.616193

Experimental method

X-RAY DIFFRACTION (3 Å)