4QY4
Crystal structure of the bromodomain of human SMARCA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 31 |
| Unit cell lengths | 64.346, 64.346, 89.117 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.730 - 1.970 |
| R-factor | 0.22633 |
| Rwork | 0.224 |
| R-free | 0.27889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ensemble of 2GRC 2oss 3d7c 2ouo 2oo1 3dai 3DWY. |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.730 | 12.740 | 2.040 |
| High resolution limit [Å] | 1.970 | 7.360 | 1.990 |
| Rmerge | 0.219 | 0.134 | 3.290 |
| Number of reflections | 28970 | ||
| <I/σ(I)> | 11.9 | 25.3 | 2.8 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.6 | 6.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 15% PEG 6K, 8% ethylene glycol, 0.01M ZnCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
