4QXU
Novel Inhibition Mechanism of Membrane Metalloprotease by an Exosite-Swiveling Conformational antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2014-03-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97624 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.560, 79.560, 93.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.260 - 2.300 |
R-factor | 0.24567 |
Rwork | 0.243 |
R-free | 0.29336 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ouu |
RMSD bond length | 0.014 |
RMSD bond angle | 1.783 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 24287 | |
Completeness [%] | 90.0 | 78.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M (NH4)2SO4, 0.01M MgCl2 0.05M MES, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |