4QQU
Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.978, 99.102, 103.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.710 - 2.980 |
R-factor | 0.18041 |
Rwork | 0.175 |
R-free | 0.27570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l5z |
RMSD bond length | 0.012 |
RMSD bond angle | 1.635 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.710 | 3.030 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.172 | 0.601 |
Number of reflections | 16414 | |
<I/σ(I)> | 5.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 50 mM sodium iodide, 27% w/v PEG3350, 0.25 mM BME, 0.15 mM zinc sulfate, 2 mM homocysteine, 5 mM 5-methyltetrahydrofolate triglutamate, 20 mM Tris-Cl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |