4QQU

Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 21
Unit cell lengths	74.978, 99.102, 103.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.710 - 2.980
R-factor	0.18041
Rwork	0.175
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l5z
RMSD bond length	0.012
RMSD bond angle	1.635
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.710	3.030
High resolution limit [Å]	2.980	2.980
Rmerge	0.172	0.601
Number of reflections	16414
<I/σ(I)>	5.4
Completeness [%]	100.0	100
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	50 mM sodium iodide, 27% w/v PEG3350, 0.25 mM BME, 0.15 mM zinc sulfate, 2 mM homocysteine, 5 mM 5-methyltetrahydrofolate triglutamate, 20 mM Tris-Cl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K