4QQU

Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase	polymer	789	88189.0	1	UniProt (P82610) Pfam (PF08267) Pfam (PF01717)	Candida albicans (Yeast)	Cobalamin-independent methionine synthase, Methionine synthase, vitamin-B12 independent isozyme
2	B (A)	2-AMINO-4-MERCAPTO-BUTYRIC ACID	non-polymer		135.2	1	Chemie (HCS)
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E (A)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	F (A)	N-[4-({[(6S)-2-amino-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-gamma-glutamyl-L-gamma-glutamyl-L-glutamic acid	non-polymer		703.7	1	BIRD (PRD_001258) Chemie (39S)
6	G (A)	water	water		18.0	41	Chemie (HOH)

Sequence modifications

A: 1 - 767 (UniProt: P82610)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Ala 103	Lys 103	engineered mutation
Ala 104	Lys 104	engineered mutation
Ala 107	Glu 107	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	88189.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1094.2
All*	Total formula weight	89283.2

*Water molecules are not included.