4QQU
Crystal structure of the cobalamin-independent methionine synthase enzyme in a closed conformation
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase | polymer | 789 | 88189.0 | 1 | UniProt (P82610) Pfam (PF08267) Pfam (PF01717) In PDB | Candida albicans (Yeast) | Cobalamin-independent methionine synthase, Methionine synthase, vitamin-B12 independent isozyme |
2 | A | 2-AMINO-4-MERCAPTO-BUTYRIC ACID | non-polymer | 135.2 | 1 | Chemie (HCS) | |||
3 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
4 | A | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
5 | A | N-[4-({[(6S)-2-amino-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-gamma-glutamyl-L-gamma-glutamyl-L-glutamic acid | non-polymer | 703.7 | 1 | BIRD (PRD_001258) Chemie (39S) | |||
6 | water | water | 18.0 | 41 | Chemie (HOH) |
Sequence modifications
A: 1 - 767 (UniProt: P82610)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
Ser -14 | - | expression tag |
Ser -13 | - | expression tag |
Gly -12 | - | expression tag |
Val -11 | - | expression tag |
Asp -10 | - | expression tag |
Leu -9 | - | expression tag |
Gly -8 | - | expression tag |
Thr -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Ser 0 | - | expression tag |
Ala 103 | Lys 103 | engineered mutation |
Ala 104 | Lys 104 | engineered mutation |
Ala 107 | Glu 107 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 88189.0 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 1094.2 | |
All* | Total formula weight | 89283.2 |