4QKX
Structure of beta2 adrenoceptor bound to a covalent agonist and an engineered nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2013-06-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.558, 67.211, 301.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.260 - 3.300 |
R-factor | 0.2401 |
Rwork | 0.236 |
R-free | 0.27950 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.634 |
Data reduction software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1241) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.300 |
High resolution limit [Å] | 3.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.8 | 293 | 24-27 % PEG400, 100 mM Tris pH 7.8 to pH 8.4, 5-10 mM Sodium formate, lipidic cubic phase, temperature 293K |