4QKD
Crystal structure of human ALKBH7 in complex with alpha-ketoglutarate and Mn(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2012-10-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.426, 82.048, 66.414 |
| Unit cell angles | 90.00, 120.03, 90.00 |
Refinement procedure
| Resolution | 33.400 - 1.350 |
| R-factor | 0.14648 |
| Rwork | 0.146 |
| R-free | 0.16275 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qkb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.348 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 124116 | |
| Completeness [%] | 91.9 | 61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 277 | PEG pH6, EVAPORATION, temperature 277K |
