4QKB
Crystal structure of seleno-methionine labelled human ALKBH7 in complex with alpha-ketoglutarate and Mn(II)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 130 |
Detector technology | CCD |
Collection date | 2013-05-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929, 0.97943 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.737, 82.759, 66.724 |
Unit cell angles | 90.00, 119.95, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.1589 |
Rwork | 0.158 |
R-free | 0.18103 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.152 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 17632 | |
Completeness [%] | 90.5 | 51 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 277 | PEG pH6, EVAPORATION, temperature 277K |