4QK4
Crystal structure of human nuclear receptor sf-1 (nr5a1) bound to pip2 at 2.8 a resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002+ |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-16 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.000, 75.000, 138.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.119 - 2.810 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.24080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yow |
RMSD bond length | 0.002 |
RMSD bond angle | 0.540 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.119 | 2.910 | |
High resolution limit [Å] | 2.810 | 6.000 | 2.810 |
Rmerge | 0.042 | 0.019 | 0.656 |
Number of reflections | 10167 | 1869 | 1825 |
<I/σ(I)> | 23.72 | 56.4 | 2.8 |
Completeness [%] | 99.4 | 97.3 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | SF-1, 6% PEG 8000, 0.2M MGOAC, 20% ETHYLENE GLYCOL, 0.067MM PIP2, 0.80MM 14-MER EEPSLLKKLLLAPA, NANODROP, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K |