4QGT
The Crystal Structure of Human IgG Fc Domain with Enhanced Aromatic Sequon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2013-11-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.072, 49.190, 75.536 |
Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
Resolution | 46.852 - 2.990 |
R-factor | 0.2992 |
Rwork | 0.298 |
R-free | 0.32620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ave |
RMSD bond length | 0.004 |
RMSD bond angle | 0.846 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.990 |
<I/σ(I)> | 12.9 |
Completeness [%] | 88.5 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100mM Hepes, 30% PEG 1000 Cryo: +10% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298KK |