4QGT

The Crystal Structure of Human IgG Fc Domain with Enhanced Aromatic Sequon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	200
Detector technology	PIXEL
Collection date	2013-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	154.072, 49.190, 75.536
Unit cell angles	90.00, 104.21, 90.00

Refinement procedure

Resolution	46.852 - 2.990
R-factor	0.2992
Rwork	0.298
R-free	0.32620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ave
RMSD bond length	0.004
RMSD bond angle	0.846
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.990
<I/σ(I)>	12.9
Completeness [%]	88.5
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100mM Hepes, 30% PEG 1000 Cryo: +10% ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298KK