4QEX
Crystal structure of PfEBA-175 RII in complex with a Fab fragment from inhibitory antibody R217
Replaces: 4K4MExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-04-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.100, 101.260, 117.590 |
| Unit cell angles | 90.00, 102.86, 90.00 |
Refinement procedure
| Resolution | 19.842 - 4.500 |
| R-factor | 0.2348 |
| Rwork | 0.232 |
| R-free | 0.28550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.600 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 13322 | |
| Completeness [%] | 92.8 | 52.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.3 M magnesium chloride, 8% benzamidine hydrochloride, 7.5% glycerol, 18% PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
