4QE8

FXR with DM175 and NCoA-2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 1
Unit cell lengths	34.483, 48.683, 82.201
Unit cell angles	88.89, 78.10, 85.07

Refinement procedure

Resolution	80.440 - 2.620
R-factor	0.252
Rwork	0.249
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.162
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	80.440
High resolution limit [Å]	2.620
Number of reflections	15585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	277	300 mM MgCl2, 100 mM Bis-Tris, 15-20% PEG 3350, pH 7, VAPOR DIFFUSION, temperature 277K