4Q4F
Crystal structure of LIMP-2 (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.686, 63.386, 114.375 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.21979 |
| Rwork | 0.217 |
| R-free | 0.26957 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q4b |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.232 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.119 | 0.378 |
| Number of reflections | 15244 | |
| <I/σ(I)> | 7.1 | 1.8 |
| Completeness [%] | 96.2 | 91.5 |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 30 %w/v Polyethylene Glycol 8000, 0.2 M Ammonium Sulphate, 0.1 M Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
