4PXU
Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 138.648, 138.648, 105.792 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.024 - 2.601 |
| R-factor | 0.2244 |
| Rwork | 0.223 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pxt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.862 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.024 | 2.740 |
| High resolution limit [Å] | 2.601 | 2.600 |
| Rmerge | 0.099 | 0.989 |
| Number of reflections | 15885 | |
| <I/σ(I)> | 10.8 | 2.3 |
| Completeness [%] | 83.8 | 99.9 |
| Redundancy | 8.2 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 4-6 % (+/-) 2-Methyl-2,4 pentanediol (MPD), 100mM MES, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
