4PWC
Phl p 4 I153V N158H variant, a glucose oxidase, 3.5 M NaBr soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Detector technology | PIXEL |
Collection date | 2014-02-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.915345 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 117.220, 117.220, 201.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.305 - 2.300 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tsh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (mr) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 68911 | |
<I/σ(I)> | 1.47 | 1.47 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 21.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 7 mg/mL Protein in 20mM Tris + 70% Tacsimate. Crystals were soaked in 55% Tacsimate 20% Glycerol 3.5 M NaBr before freezing, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |