4PVF

Crystal structure of Homo sapiens holo serine hydroxymethyltransferase 2 (mitochondrial) (SHMT2), isoform 3, transcript variant 5, 483 aa, at 2.6 ang. resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	P 65 2 2
Unit cell lengths	160.462, 160.462, 211.461
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	138.960 - 2.600
R-factor	0.2077
Rwork	0.206
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3OU5
RMSD bond length	0.014
RMSD bond angle	1.747
Data reduction software	XDS
Data scaling software	SCALA (0.1.29)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	138.964	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	49990
<I/σ(I)>		1.8
Completeness [%]	100.0	100
Redundancy	15.4	15.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.1M Bis-Tris propane pH7.5, 5%glycerol, 12-16% Peg 3350, 0.2M KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K