4PV0
Crystal structure of spleen tyrosine kinase (Syk) in complex with an imidazopyrazine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.788, 84.972, 90.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.486 - 2.000 |
| R-factor | 0.2306 |
| Rwork | 0.228 |
| R-free | 0.28200 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1xba |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.490 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.581 |
| Number of reflections | 20675 | |
| Completeness [%] | 96.7 | 97.8 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
