4PNI
Bovine G protein-coupled receptor kinase 1 in complex with GSK2163632A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.485, 66.566, 205.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.670 - 1.850 |
R-factor | 0.19054 |
Rwork | 0.188 |
R-free | 0.23024 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.004 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 51503 | |
<I/σ(I)> | 90 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.3 | 19.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | MES, PEG 3350, NaCl |