4PH7

Structure of Osh6p in complex with phosphatidylinositol 4-phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97298
Spacegroup name	P 21 21 21
Unit cell lengths	114.110, 122.260, 141.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.562 - 2.550
R-factor	0.1905
Rwork	0.188
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b2z
RMSD bond length	0.003
RMSD bond angle	0.727
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (phenix.mr)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.560	2.690
High resolution limit [Å]	2.550	2.550
Number of reflections	77182
<I/σ(I)>	15.65	4.95
Completeness [%]	100.0	100
Redundancy	8.2	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	200 mM Ammonium Acetate, 100 mM BisTris, 20% PEG 3350