4PGZ

Structural basis of KIT activation by oncogenic mutations in the extracellular region reveals a zipper-like mechanism for ligand-dependent or oncogenic receptor tyrosine kinase activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	110
Detector technology	PIXEL
Collection date	2011-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.10000
Spacegroup name	C 1 2 1
Unit cell lengths	163.592, 63.548, 81.547
Unit cell angles	90.00, 117.46, 90.00

Refinement procedure

Resolution	42.390 - 2.400
R-factor	0.2334
Rwork	0.232
R-free	0.26170
RMSD bond length	0.002
RMSD bond angle	0.657
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.108	0.080	0.824
Total number of observations	178278
Number of reflections	29387
<I/σ(I)>	8.3
Completeness [%]	99.9	100	98.7
Redundancy	6.1	6	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	10%-18% [w/v] PEG 3350, 20 mM cobalt chloride