4PF9
Crystal structure of insulin degrading enzyme complexed with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-11 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.254, 116.419, 124.470 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.500 |
| R-factor | 0.1955 |
| Rwork | 0.195 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g47 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.658 | |
| Number of reflections | 71156 | |
| <I/σ(I)> | 6.8 | 1.7 |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 20% PEG3350, 0.2 mM sodium thiocyanate |
