4PEH
Dbr1 in complex with synthetic linear RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.021, 141.936, 212.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.033 - 2.100 |
R-factor | 0.2056 |
Rwork | 0.203 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.907 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.980 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.521 | |
Number of reflections | 129196 | |
<I/σ(I)> | 11 | 2.8 |
Completeness [%] | 99.5 | 99.4 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M lithium sulfate, 0.1 M bis-tris, 25% PEG 3350, 8.5 % glycerol, 3.0 mM manganese sulfate, 1.45 mM AK65 |