4PE5
Crystal Structure of GluN1a/GluN2B NMDA Receptor Ion Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.825, 163.186, 163.137 |
| Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
| Resolution | 29.717 - 3.960 |
| R-factor | 0.2583 |
| Rwork | 0.256 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3QEL 2a5t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.090 |
| High resolution limit [Å] | 3.950 | 8.500 | 3.950 |
| Rmerge | 0.087 | 0.050 | |
| Total number of observations | 197482 | ||
| Number of reflections | 52241 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 98.6 | 90.9 | 99.4 |
| Redundancy | 3.8 | 3.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 291 | PEG 4000, Tris-HCl, NaCl, Magnesium acetate, metatungstate |
