4PAO

A conserved phenylalanine as relay between the 5 helix and the GDP binding region of heterotrimeric G protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 32 2 1
Unit cell lengths	79.177, 79.177, 104.885
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.000 - 2.003
R-factor	0.1686
Rwork	0.164
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gia
RMSD bond length	0.007
RMSD bond angle	0.981
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	2.070
High resolution limit [Å]	2.000	2.000
Total number of observations		100
Number of reflections	26186
<I/σ(I)>	13.5	3.05
Completeness [%]	100.0
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	(NH4)2SO3, Sodium acetate