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4PAN

A conserved phenylalanine as relay between the 5 helix and the GDP binding region of heterotrimeric G protein

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-G
Synchrotron siteAPS
Beamline21-ID-G
Temperature [K]100
Detector technologyCCD
Collection date2013-07-07
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.978
Spacegroup nameI 4
Unit cell lengths121.533, 121.533, 68.279
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.400
R-factor0.1872
Rwork0.182
R-free0.23220
RMSD bond length0.008
RMSD bond angle1.011
Refinement softwarePHENIX ((phenix.refine: 1.8.2_1309))
Data quality characteristics
 Overall
Low resolution limit [Å]20.000
High resolution limit [Å]2.400
Number of reflections19424
<I/σ(I)>19.3
Completeness [%]99.2
Redundancy4.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP291(NH4)2SO3, Sodium acetate

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PDB entries from 2024-09-18

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