4P4H

Caught-in-action signaling complex of RIG-I 2CARD domain and MAVS CARD domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	111.810, 117.330, 257.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 3.400
R-factor	0.1936
Rwork	0.191
R-free	0.23970
RMSD bond length	0.011
RMSD bond angle	1.268
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	3.438
High resolution limit [Å]	3.400	3.400
Rmerge	0.160	1.315
Number of reflections	89450
<I/σ(I)>	8.28	1.26
Completeness [%]	99.1	97.8
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	Tri-Li Citrate, PEG 3350, Cr(III) Cl3