4P42

Extended-Synaptotagmin 2, SMP - C2A - C2B Domains

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792, 1.907
Spacegroup name	P 21 21 21
Unit cell lengths	74.332, 88.382, 167.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.140 - 2.440
R-factor	0.2124
Rwork	0.210
R-free	0.25560
RMSD bond length	0.003
RMSD bond angle	0.846
Refinement software	PHENIX ((phenix.refine: dev_1452))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.140
High resolution limit [Å]	2.440
Rmerge	0.089
Number of reflections	41769
<I/σ(I)>	2.4
Completeness [%]	98.9
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 1500, MIB Buffer