4OZT

crystal structure of the ligand binding domains of the Bovicola ovis ecdysone receptor EcR/USP heterodimer (PonA crystal)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.9536
Spacegroup name	P 43 21 2
Unit cell lengths	134.000, 134.000, 95.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.830 - 2.700
R-factor	0.184
Rwork	0.182
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.418
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.800
High resolution limit [Å]	2.700
Number of reflections	24458
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350 with either 0.2 M potassium acetate or 0.2 M sodium citrate