4OZ2

Human solAC Complexed with 4-(4-Fluorophenyl)-3-methyl-1H-pyrazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93300
Spacegroup name	P 63
Unit cell lengths	99.436, 99.436, 97.604
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.540 - 2.100
R-factor	0.1686
Rwork	0.166
R-free	0.22150
RMSD bond length	0.012
RMSD bond angle	1.120
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.540
High resolution limit [Å]	2.100
Number of reflections	30815
<I/σ(I)>	11.7
Completeness [%]	96.2
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1ul of protein solution was mixed with 1ul of reservoir solution (0.1M sodium acetate, pH 4.8, 0.2M trisodium citrate, 16-18% PEG4K and 10% glycerol) and left to equilibrate at 4C