4OT1
Structural Basis for the Recognition of Human Cytomegalovirus Glycoprotein B by the Neutralizing Human Antibody SM5-1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.071, 69.411, 100.991 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 42.460 - 2.110 |
| R-factor | 0.17434 |
| Rwork | 0.171 |
| R-free | 0.23051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.844 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.460 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Number of reflections | 31875 | |
| Completeness [%] | 98.5 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M L-Proline, 0.1M HEPES, 24% (w/v) Polyethylene glycol 1500, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
