4ONG
Fab fragment of 3D6 in complex with amyloid beta 1-40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-14 |
| Detector | MAR |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 40.040, 84.940, 175.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.474 - 2.200 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3D6 FAB ANTIBODY |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | COMO |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 87.845 | 87.845 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.059 | 0.031 | 0.189 |
| Total number of observations | 2049 | 13424 | |
| Number of reflections | 31474 | ||
| <I/σ(I)> | 19.9 | 15.7 | 3.8 |
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 5.8 | 4.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 2.5 M NaCl, 0.1 Imidazole pH 8.0, 0.2 ZnAc2., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
