4OMP
Crystal structure of the intertwined dimer of the c-Src tyrosine kinase SH3 domain mutant Q128K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-11 |
Detector | PILATUS 6M (DECTRIS) |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 46.862, 46.862, 127.849 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.390 - 2.000 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jz4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.141 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.390 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.530 |
Number of reflections | 6002 | |
<I/σ(I)> | 23.4 | 4.9 |
Completeness [%] | 98.6 | 96.3 |
Redundancy | 11.7 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5 | 298 | 0.1M sodium acetate, 1.8 M ammonium sulphate, 5% PEG 300, 10%Glicerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |