4OMP
Crystal structure of the intertwined dimer of the c-Src tyrosine kinase SH3 domain mutant Q128K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-11 |
| Detector | PILATUS 6M (DECTRIS) |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 46.862, 46.862, 127.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.390 - 2.000 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jz4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.530 |
| Number of reflections | 6002 | |
| <I/σ(I)> | 23.4 | 4.9 |
| Completeness [%] | 98.6 | 96.3 |
| Redundancy | 11.7 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 0.1M sodium acetate, 1.8 M ammonium sulphate, 5% PEG 300, 10%Glicerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
