4OMN
Crystal structure of the intertwined dimer of the c-Src tyrosine kinase SH3 domain mutant Q128E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 46.688, 46.688, 127.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.550 - 1.500 |
| R-factor | 0.157 |
| Rwork | 0.156 |
| R-free | 0.16800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jz4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.30) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.560 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.708 |
| Number of reflections | 14008 | |
| <I/σ(I)> | 26.9 | 4.6 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 12.1 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 0.1M sodium acetate, 1.8 M ammonium sulphate, 10%PEG 200, 10%Glicerol, 10mM NDSB, pH 5.0, vapor diffusion, hanging drop, temperature 298K |
