4OG6
Human menin with bound inhibitor MIV-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Detector technology | CCD |
| Collection date | 2012-08-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.568, 79.865, 124.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.020 - 1.490 |
| R-factor | 0.16202 |
| Rwork | 0.161 |
| R-free | 0.18819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gpq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.816 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.020 |
| High resolution limit [Å] | 1.490 |
| Rmerge | 0.069 |
| Number of reflections | 74065 |
| <I/σ(I)> | 28.38 |
| Completeness [%] | 98.5 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 283 | 0.2 M ammonium acetate, 0.1 M HEPES pH 7.5 and 25% w/v PEG 3,350. This solution was mixed 1:1 with 2.5mg/mL protein in 50mM Tris-HCl (pH 8.0), 50mM NaCl, and 1mM TCEP. Prior to data collection, crystals were transferred into a cryo-solution containing 20% PEG550 MME and flash-frozen in liquid nitrogen, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
