4ODO
Structure of SlyD from Thermus thermophilus in complex with FK506
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.710, 50.210, 57.670 |
| Unit cell angles | 85.74, 68.92, 80.12 |
Refinement procedure
| Resolution | 27.810 - 1.599 |
| R-factor | 0.1808 |
| Rwork | 0.180 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.899 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.810 | 1.640 |
| High resolution limit [Å] | 1.599 | 1.599 |
| Number of reflections | 63368 | |
| <I/σ(I)> | 14.46 | 2.47 |
| Completeness [%] | 95.3 | 87.4 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% PEG3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
