4OC7
Retinoic acid receptor alpha in complex with (E)-3-(3'-allyl-6-hydroxy-[1,1'-biphenyl]-3-yl)acrylic acid and a fragment of the coactivator TIF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.554, 67.554, 110.075 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.768 - 2.500 |
R-factor | 0.1999 |
Rwork | 0.198 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.750 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.770 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 9327 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 98.8 | 100 |
Redundancy | 25.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Na HEPES pH 7.5, 20% PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K |