4O9M
Human DNA polymerase beta complexed with adenylated tetrahydrofuran (abasic site) containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-21 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.627, 78.873, 54.324 |
Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
Resolution | 23.510 - 2.295 |
R-factor | 0.2002 |
Rwork | 0.193 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2p66 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.204 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.510 |
High resolution limit [Å] | 2.295 |
Number of reflections | 19178 |
Completeness [%] | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 100 mM Bis-Tris, 350 mM Sodium Acetate, 16% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |