4O9M

Human DNA polymerase beta complexed with adenylated tetrahydrofuran (abasic site) containing DNA

Summary for 4O9M

• Entry DOI: 10.2210/pdb4o9m/pdb
• Descriptor: DNA (5'-D(*CP*CP*GP*AP*CP*AP*GP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*CP*C)-3'), (5'-D(P*GP*TP*CP*GP*G)-3');, ... (7 entities in total)
• Functional Keywords: lyase, aprataxin, adenylated tetrahydrofuran, transferase-dna complex, transferase/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P06746
• Total number of polymer chains: 4
• Total formula weight: 48554.35

Authors

Caglayan, M.,Batra, V.K.,Sassa, A.,Prasad, R.,Wilson, S.H. (deposition date: 2014-01-02, release date: 2014-04-30, Last modification date: 2023-09-20)

Primary citation

Caglayan, M.,Batra, V.K.,Sassa, A.,Prasad, R.,Wilson, S.H.
Role of polymerase beta in complementing aprataxin deficiency during abasic-site base excision repair.
Nat.Struct.Mol.Biol., 21:497-499, 2014

PubMed Abstract: DNA polymerase β (pol β) lyase removal of 5'-deoxyribose phosphate (5'-dRP) from base excision repair (BER) intermediates is critical in mammalian BER involving the abasic site. We found that pol β also removes 5'-adenylated dRP from BER intermediates after abortive ligation. The crystal structure of a human pol β-DNA complex showed the 5'-AMP-dRP group positioned in the lyase active site. Pol β expression rescued methyl methanesulfonate sensitivity in aprataxin (hnt3)- and FEN1 (rad27)-deficient yeast.

PubMed: 24777061
DOI: 10.1038/nsmb.2818

Experimental method

X-RAY DIFFRACTION (2.295 Å)