4O6H
2.8A crystal structure of Lymphocytic Choriomeningitis Virus Nucleoprotein C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.856, 94.403, 145.124 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 47.264 - 2.800 |
| R-factor | 0.2287 |
| Rwork | 0.227 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q7c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.676 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.264 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 58941 |
| Completeness [%] | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.35M potassium phosphate, 20% PEG 3350, cryoprotected with 20% glycerol, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
