4O68

Structure of human cyclic GMP-AMP synthase (cGAS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97899
Spacegroup name	P 21 21 2
Unit cell lengths	162.194, 47.051, 58.404
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.675 - 2.436
R-factor	0.1839
Rwork	0.180
R-free	0.24380
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.251
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.450	2.450
Number of reflections	16956
Completeness [%]	97.5	83.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	6% PEG 3,350, 0.1 M Hepes, pH 7.8, 0.05 M NaCl, 0.01 M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K