4O5I

Crystal structure of broadly neutralizing antibody F045-092 in complex with A/Victoria/361/2011 (H3N2) influenza hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	77
Detector technology	CCD
Collection date	2013-02-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	C 1 2 1
Unit cell lengths	318.123, 187.167, 353.636
Unit cell angles	90.00, 90.47, 90.00

Refinement procedure

Resolution	49.000 - 6.501
R-factor	0.2032
Rwork	0.201
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	apo F045-092 structure apo A/Victoria/361/2011 (H3N2) HA structure
RMSD bond length	0.006
RMSD bond angle	1.218
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.890
High resolution limit [Å]	6.500	6.500
Rmerge	0.070	0.500
Number of reflections	38102
<I/σ(I)>	10.8	2.1
Completeness [%]	92.8	91.7
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG 400, 30% 1,2-propanediol, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K