4O58
Crystal structure of broadly neutralizing antibody F045-092 in complex with A/Victoria/3/1975 (H3N2) influenza hemagglutinin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-04-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03322 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 99.101, 99.101, 336.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.022 - 2.750 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GMS and apo F045-092 structure |
RMSD bond length | 0.010 |
RMSD bond angle | 1.253 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.920 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.100 | 0.710 |
Number of reflections | 50811 | |
<I/σ(I)> | 13.3 | 2.3 |
Completeness [%] | 99.5 | 97.7 |
Redundancy | 6.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 277 | 1.7 M ammonium sulfate, 0.1 M Tris pH 8.1, 4% PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |